Restriction note
Declared a noxious or prohibited species across much of Australia and restricted in a number of other jurisdictions. In parts of its introduced range it is instead actively distributed by public health bodies, so the legal position is genuinely contradictory between countries and must be checked locally rather than assumed.
What AquariumHQ has recorded, by jurisdiction, with the instrument each entry comes from and how far the check went. Ecological risk and legal status are separate: a species can be ecologically risky and legally unrestricted, or restricted where it could never establish.
EU
ProhibitedpossessionGambusia holbrooki Girard, 1859 appears by name in the Union list, added by the 2022 amendment, alongside its western congener Gambusia affinis. Article 7 prohibits keeping, breeding, transporting, placing on the market, using, exchanging and releasing either.
Instrument: Regulation (EU) No 1143/2014, Article 7, as applied to the Union list in Commission Implementing Regulation (EU) 2016/1141 (consolidated text 02016R1141-20220802)
Both Gambusia species are listed, which matters because the two are difficult to distinguish and are used interchangeably in mosquito-control programmes. A keeper cannot escape the listing by arguing about which species they have.
Checked against the instrument · last checked 2026-08-29 · An editor has read the current text of the named instrument and confirmed this species' entry on the date shown.
AU
ProhibitedpossessionThe species is declared noxious across much of Australia, where possession and movement of live fish are offences under state law. The specific declarations differ between states and territories and have not been read individually here.
Instrument: State and territory noxious-fish declarations made under fisheries and biosecurity legislation
The legal position on this species is genuinely contradictory between countries. In parts of its introduced range public health bodies still distribute it deliberately for mosquito control, while in Australia and the EU possession is an offence. Neither position can be inferred from the other.
Instrument recorded, species entry not re-checked · last checked 2026-08-29 · AquariumHQ has recorded the instrument and what it does, but has not re-confirmed this species' entry against the current consolidated text. Listings are amended, so treat this as a pointer to the right law rather than as a confirmation of the current position, and check with the authority before acting.
JP
Permit requiredpossessionBoth Gambusia affinis and G. holbrooki are designated by name. A keeping permit under the Act may be granted for academic research, exhibition, education and similar purposes. The Ministry states directly that keeping as a pet is not among them, so for an aquarist the practical effect of “permit required” is prohibition.
Instrument: Act on the Prevention of Adverse Ecological Effects Caused by Designated Invasive Alien Species (Act No. 78 of 2004), as amended 2022 — designated invasive alien species, Gambusia holbrooki
Checked against the instrument · last checked 2026-08-30 · An editor has read the current text of the named instrument and confirmed this species' entry on the date shown.
JP
ProhibitedreleaseRelease is prohibited. Gambusia were introduced to Japan and to much of the rest of the world deliberately, as a mosquito-control measure, which is the historical reason the genus is now on prohibition lists across four continents.
Instrument: Act on the Prevention of Adverse Ecological Effects Caused by Designated Invasive Alien Species (Act No. 78 of 2004), as amended 2022 — designated invasive alien species, Gambusia holbrooki
Checked against the instrument · last checked 2026-08-30 · An editor has read the current text of the named instrument and confirmed this species' entry on the date shown.
Legal status is recorded from the sources named and was correct on the verification date shown. Rules change, and sub-national rules often differ from national ones. Check the current position with the relevant authority before acquiring, selling or moving any animal or plant.
Group size and social behaviour
Aggregates loosely. Females are dominant and larger, and both sexes harass tankmates persistently.